Tobacco toxins deposited on surfaces (third hand smoke) impair wound healing.

TitleTobacco toxins deposited on surfaces (third hand smoke) impair wound healing.
Publication TypeJournal Article
Year of Publication2016
AuthorsDhall S, Alamat R, Castro A, Sarker AH, Mao J-H, Chan A, Hang B, Martins-Green M
JournalClin Sci (Lond)
Volume130
Issue14
Pagination1269-84
Date Published2016 Jul 1
ISSN1470-8736
Abstract

Third hand smoke (THS) is the accumulation of second hand smoke (SHS) toxins on surfaces in homes, cars, clothing and hair of smokers. It is known that 88M US nonsmokers ≥3 years old living in homes of smokers are exposed to THS toxicants and show blood cotinine levels of ≥0.05 ng/ml, indicating that the toxins are circulating in their circulatory systems. The goal of the present study is to investigate the mechanisms by which THS causes impaired wound healing. We show that mice living under conditions that mimic THS exposure in humans display delayed wound closure, impaired collagen deposition, altered inflammatory response, decreased angiogenesis, microvessels with fibrin cuffs and a highly proteolytic wound environment. Moreover, THS-exposed mouse wounds have high levels of oxidative stress and significantly lower levels of antioxidant activity leading to molecular damage, including protein nitration, lipid peroxidation and DNA damage that contribute to tissue dysfunction. Furthermore, we show that elastase is elevated, suggesting that elastin is degraded and the plasticity of the wound tissue is decreased. Taken together, our results lead us to conclude that THS toxicants delay and impair wound healing by disrupting the sequential processes that lead to normal healing. In addition, the lack of elastin results in loss of wound plasticity, which may be responsible for reopening of wounds.

DOI10.1042/CS20160236
Alternate JournalClin. Sci.
PubMed ID27129193