TY - JOUR T1 - Transgenic mice for cre-inducible overexpression of the Cul4A gene. JF - Genesis Y1 - 2011 A1 - Li, Tong A1 - Hung, Ming-Szu A1 - Wang, Yucheng A1 - Jiang-Hua Mao A1 - Tan, Jia-Li A1 - Jahan, Kenneth A1 - Roos, Hannah A1 - Xu, Zhidong A1 - Jablons, David M A1 - You, Liang KW - Adenoviridae KW - Animals KW - Cullin Proteins KW - Gene Expression Regulation KW - Genes, Reporter KW - Genetic Vectors KW - Integrases KW - Lac Operon KW - Mice KW - Mice, Knockout KW - Mice, Transgenic KW - Models, Animal KW - Promoter Regions, Genetic KW - Recombination, Genetic KW - Transgenes AB -

The Cullin 4A(Cul4A) gene is important in cell survival, development, growth, and cell cycle control and is amplified in breast and hepatocellular cancers. Recently, we reported that Cul4A plays an oncogenic role in the pathogenesis of mesothelioma. An important strategy for studying Cul4A in different tissues is targeted overexpression of this gene in vivo. Studies of Cul4A in mice have been restricted to the loss-of-function studies using Cul4A knockout mice; gain-of-function studies of Cul4A using transgenic mice have not been reported. We, therefore, generated a gain-of-function transgenic mouse model that overexpresses Cul4A in a Cre-dependent manner. Before Cre recombination, these mice express LacZ during development in most adult tissues. After Cre-mediated excision of the LacZ reporter, the transfected Cul4A gene is expressed along with a C-terminal Myc-tag in different tissues. In this study, Cre-excision was induced in mouse lungs by inhalation of an adenovirus vector encoding Cre recombinase. This mouse model provides a valuable resource for investigating the significance of Cul4A overexpression in various tissues.

VL - 49 IS - 3 U1 - http://www.ncbi.nlm.nih.gov/pubmed/21381181?dopt=Abstract ER -