@article {270,
	title = {Increased gene copy numbers at chromosome 20q are frequent in both squamous cell carcinomas and adenocarcinomas of the cervix.},
	journal = {J Pathol},
	volume = {209},
	year = {2006},
	month = {2006 Jun},
	pages = {220-30},
	abstract = {<p>Genome-wide microarray-based comparative genomic hybridization (array CGH) was used to identify common chromosomal alterations involved in cervical carcinogenesis as a first step towards the discovery of novel biomarkers. The genomic profiles of nine squamous cell carcinomas (SCCs) and seven adenocarcinomas (AdCAs), as well as four human papillomavirus (HPV)-immortalized keratinocyte cell lines, were assessed. On a genome-wide scale, SCCs showed significantly more gains than AdCAs. More specifically, there was a striking and highly significant difference between the two histological types for gain at 3q12.1-28, which was predominantly observed in SCC. Other frequent alterations included gains of 1q21.1-31.1 and 20q11.21-13.33, and losses of 11q22.3-25 and 13q14.3-21.33. Subsequent FISH analysis for hTR, located at 3q26, confirmed the presence of 3q gain in SCCs and HPV-immortalized cell lines. Fine mapping of chromosome 20q using multiplex ligation-dependent probe amplification (MLPA) showed copy number increases for a number of genes located at 20q11-q12, including DNMT3B and TOP1. For DNMT3B, this correlated with elevated mRNA expression in 79\% of cases. In conclusion, the assessment of frequent genomic alterations resulted in the identification of potential novel biomarkers, which may ultimately enable a better risk stratification of high-risk (hr)-HPV-positive women.</p>
},
	keywords = {Adenocarcinoma, Adult, Aged, Carcinoma, Squamous Cell, Cell Line, Tumor, Chromosome Aberrations, Chromosome Mapping, Chromosomes, Human, Chromosomes, Human, Pair 20, DNA (Cytosine-5-)-Methyltransferase, Female, Genome, Human, Humans, In Situ Hybridization, Fluorescence, Middle Aged, Oligonucleotide Array Sequence Analysis, Papillomaviridae, RNA, Messenger, RNA, Neoplasm, Uterine Cervical Neoplasms},
	issn = {0022-3417},
	doi = {10.1002/path.1966},
	author = {Wilting, S M and Snijders, P J F and Meijer, G A and Ylstra, B and van den Ijssel, P R L A and A Snijders and Albertson, D G and Coffa, J and Schouten, J P and van de Wiel, M A and Meijer, C J L M and Steenbergen, R D M}
}
